Education & Training
- Ph.D. from California Institute of Technology, Pasadena, CA, 2005
- M.S. from Seoul National University, Seoul, Korea, 1997
- B.A. from Seoul National University, Seoul, Korea, 1995
Research Interest Summary
Our long-term goal is to completely understand the molecular and cellular mechanisms underlying BEC-driven liver regeneration. As a first step in pursuit of that goal, we recently established and reported an innovative zebrafish liver regeneration model in which hepatocyte-specific ablation results in extensive BEC-driven liver regeneration. Using this model, we found that Wnt, Bmp and Fgf signaling play important roles in the regeneration process. Moreover, we identified candidate genes, which are upregulated during liver regeneration and may regulate liver regeneration, by comparing gene expression profiles during liver regeneration. We have been determining in detail the roles of the signaling pathways and the candidate genes in BEC-driven liver regeneration.
In addition to the liver regeneration studies, we study biliary morphogenesis. We recently found that proper biliary morphogenesis needs proper Wnt/β-catenin signaling. We have been investigating how Wnt/β-catenin signaling regulates biliary morphogenesis at the cellular and molecular levels. Moreover, as collaboration with Dr. Sindhi, a pediatric liver transplant surgeon at the Children’s Hospital of Pittsburgh, we discovered that Arf6, whose SNP variation is significantly associated with biliary atresia patients, is required for proper biliary morphogenesis. We have been investigating the molecular and cellular mechanisms by which Arf6 regulates biliary morphogenesis.
Shin D, Monga SP (2013), Cellular and Molecular Basis of Liver Development. Comprehensive Physiology, 3(2):799-815. PMCID: PMC
Choi TY, Ninov N, Stainier DY, Shin D (2014), Extensive conversion of hepatic biliary epithelial cells to hepatocytes after near total loss of hepatocytes in zebrafish. Gastroenterology, 146(3):776-788. PMCID: PMC3943869
Delgado ER, Yang J, So J, Skoda E, Leimgruber S, Kahn M, Ishitani T, Shin D, Wilson GM, Monga SP (2014), Identification and characterization of a novel small molecule inhibitor of beta-catenin signaling. Am J Pathol, 184(7):2111-22. PMCID: PMC4076560
Shin D, Lee Y, Poss KD, Stainier DY (2011), Restriction of hepatic competence by Fgf signaling. Development, 138:1339-48. PMCID: PMC3050664
Shin D, Weidinger G, Moon RT, Stainier DY (2012), Intrinsic and extrinsic modifiers of the regulative capacity of the developing liver. Mech Dev, 128:525-35. PMCID: PMC3297115
So J, Martin BL, Kimelman D, Shin D (2013), Wnt/β-catenin signaling cell-autonomously converts non-hepatic endodermal cells to a liver fate. Biol Open, 2:30-6. PMCID: PMC3545266
Shin D, Garcia-Cardena G, Hayashi S, Gerety S, Asahara T, Stavrakis G, Isner J, Folkman J, Gimbrone MA Jr, Anderson DJ (2001), Expression of ephrinB2 identifies a stable genetic difference between arterial and venous vascular smooth muscle as well as endothelial cells, and marks subsets of microvessels at sites of adult neovascularization. Dev Biol, 230(2):139-50.
Mukouyama YS, Shin D, Britsch S, Taniguchi M, Anderson DJ (2002), Sensory nerves determine the pattern of arterial differentiation and blood vessel branching in the skin. Cell, 109(6):693-705.
Shin D, Anderson DJ (2005), Isolation of arterial-specific genes by subtractive hybridization reveals molecular heterogeneity among arterial endothelial cells. Dev Dyn, 233(4):1589-604.
Shin D, Shin CH, Tucker J, Ober EA, Rentzsch F, Poss KD, Hammerschmidt M, Mullins MC, Stainier DY (2007), Bmp and Fgf signaling are essential for liver specification in zebrafish. Development, 134:2041-50.